FastQCFastQC Report
Fri 10 Feb 2017
ERR1148308.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148308.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1699346
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT203571.197931439506728No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT124840.7346355597977104No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93900.5525655163810077No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA69280.40768625106364453No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT59340.34919316019221514No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51620.3037639185898575No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG37800.22243851458149194No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT28300.16653465509672546No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG27800.16359234670279035No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA26390.15529503703189343No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA23940.14087772590161154No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG23660.1392300332010079No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21770.12810810747193332No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC21490.1264604147713297No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT20910.12304733703436498No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20320.11957541312952158No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAA19390.11410271951680234No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC17920.10545233283863321No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG1850.054.6561972
TATAACG2000.044.60912
TCACGCA2850.035.478584
CGCATAG2600.034.3429871
ATCACGC3150.032.099673
ATGGGGG30500.030.226935
GGTTTCA9150.029.9265671
CATGGGG55200.029.0928924
CATGGGT25350.028.1556434
TTAACGC3350.026.63234
TGGGGGG25800.026.511876
GTATAAC4050.026.4568181
AACGCAG327750.025.9510886
GTATCAA335050.024.9268971
ATTAACG3351.8189894E-1224.8568133
TACAACG2651.3860699E-924.6893122
GGTTAAG9450.023.2879963
ACGCAGA366050.023.2033147
CGCAGAG366400.023.1649158
TAACGCA4700.022.7791164
Produced by FastQC (version 0.11.5)