FastQCFastQC Report
Fri 10 Feb 2017
ERR1148307.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148307.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2937216
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT301491.0264481740532532No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA176250.6000580141194928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT175650.5980152634331285No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT151510.515828594151741No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT149900.5103472131433303No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG84200.28666601298644706No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79790.2716517954416699No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG72110.24550458665620775No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT62070.21132255850437967No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC57630.19620620342528436No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG56000.1906567307273282No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC53740.18296236980868957No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC48590.16542875975072993No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT44870.1527637054952717No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC43440.1478951496927703No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA42760.14558003224822416No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC41120.13999651370549526No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39810.1355365080402667No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA36090.12287145378480847No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35770.12178198675208088No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30990.10550807295071252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG3950.042.2010271
TATAACG2350.040.4969372
GGGTTAG13750.040.2663571
TATCACG2950.038.3090742
ATGGGGG73250.034.0221445
GTTAGAG14950.033.816843
GGTTAGA15400.033.2160382
TTAGAGC15650.033.0643654
TAGAGCA15850.032.647155
CATGGGG113850.032.3379824
TACAACG3550.031.8343012
GGTTAAG22050.030.7504523
GTTAAGA23800.030.2387394
TGGGGGG65000.030.0134376
GTGGTTA17650.029.6824721
AGAGCGC8900.029.4047818
ATATGTA16150.029.1216961
TAGTACT6400.028.8095865
GTACATT12150.028.4193291
AAGAGCG8700.028.0297937
Produced by FastQC (version 0.11.5)