FastQCFastQC Report
Fri 10 Feb 2017
ERR1148303.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148303.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1457028
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133300.9148760353267062No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78600.5394542863966925No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA58450.4011590717542834No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT51650.3544887263662744No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42280.2901797357360325No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG35500.24364665606975297No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27630.189632594569219No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG25820.17721004675270483No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT24370.16725828192732053No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC21840.1498941681285466No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT18660.1280689183735659No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC17390.1193525450437466No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG16650.11427371333975736No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC15670.1075476929750149No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTAGA8150.043.7885062
GGGTTAG8050.043.6205371
GTTAGAG8950.039.2112243
GGTTAAG9500.035.6888583
TTAGAGC9850.035.628474
TATCACG1703.274181E-1034.9878772
GTGGTTA8000.034.965741
GTTAAGA9900.034.2468834
TAGAGCA10100.034.1576545
ATGGGGG30450.032.0359765
GTATAGG2109.094947E-1131.1751291
CATGGGG49250.031.1598174
CGCATAG1751.6676495E-830.6083071
TACAACG2351.0913936E-1130.3724562
GTACATT5950.030.0081461
TGGGGGG25700.028.4677686
TATAACG1306.394487E-527.4520262
GTACAAC3500.027.2073841
AACGCAG236650.026.4166416
CATGGGT18450.025.7914074
Produced by FastQC (version 0.11.5)