FastQCFastQC Report
Fri 10 Feb 2017
ERR1148293.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148293.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5436409
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT489510.9004289412367613No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT265140.4877116493626583No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA257080.47288568612111415No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT250300.46041421828269363No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245380.45136412657693703No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG124140.22834926511231957No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122540.22540614585841498No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG102780.18905862307269378No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC87730.16137490759065404No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT85760.15775119200928406No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG84510.15545188009217112No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA79650.14651215535843606No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC73330.13488683430551307No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC72540.1334336691738977No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC71730.1319437150516085No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC65870.1211645407841831No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT59480.10941045826390178No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGGGGG115800.038.366765
TATCACG5750.038.2719842
CATGGGG183550.034.9307144
GGGTTAG21000.034.8700451
TGGGGGG98000.034.411226
TATAACG5950.033.9867022
GTTAGAG22400.032.1277583
TTAGAGC25300.031.0310544
CGCATAG6400.030.6973361
GGTTAGA22700.030.3933942
TACAACG8000.028.9949072
TAGAGCA24050.028.934365
GTTAAGA33900.026.6677884
ACAACGC9550.026.1570643
CATGGGT71700.026.1297054
GGTTAAG34450.024.6882273
CTATAGC14250.023.7904743
TAACGCA9600.023.5426564
GTGGTTA26300.023.5420511
ATATGTA26400.022.7763521
Produced by FastQC (version 0.11.5)