FastQCFastQC Report
Fri 10 Feb 2017
ERR1148289.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148289.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3820807
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT376220.9846610938474516No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211280.5529721862423305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT193790.5071965163380406No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA180490.4723871161249443No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT162170.4244391302675063No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95000.24863857295068817No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG86380.22607789401558362No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG72540.18985517980887284No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT64110.16779177801966966No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC59490.15570009162985726No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG58840.15399888034124729No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC50440.1320139959961338No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50300.1316475812570486No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA48080.12583729039441144No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA46820.12253955774264441No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC45550.11921565260951417No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT44960.11767147620908357No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA43530.11392881137414165No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41430.10843259028786327No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG6300.039.6536872
TATCACG4100.037.719362
ATGGGGG73250.036.13545
CATGGGG125750.032.6851544
ACAACGC8450.031.6764343
ATTAACG6450.030.4322973
TTAACGC6450.030.4322974
TGGGGGG63250.030.2813936
GGGTTAG10450.029.6270451
CATGGGT48250.028.9701734
GGTTAGA11400.028.1749862
TATAACG4650.028.1413252
TAACGCA7800.026.6903295
TATGTAA16500.026.3156242
ATATGTA16800.026.2254961
CTATAGC10250.025.533443
GTTAGAG13100.025.4271223
GTAAGTA26300.024.8777872
GTACAAC13750.024.6816061
CGCATAG6100.024.4012171
Produced by FastQC (version 0.11.5)