FastQCFastQC Report
Fri 10 Feb 2017
ERR1148287.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148287.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4435291
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT342900.7731172543131893No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA198850.44833585890982125No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198560.4476820122963747No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT178550.40256659596856215No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT174550.3935480219899889No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG93940.2118012098867921No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT88540.19962613501571824No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG80120.18064203679082164No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT77180.1740133849165703No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC68180.15372159346478056No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG62520.14096031128509945No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA59480.1341061950613838No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC58500.13189664443663338No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC57140.12883032928391847No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC56260.12684624300863234No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT48990.11045498480257554No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47400.10687010164609266No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG5450.043.6535152
ATGGGGG87200.033.7636455
TACAACG5700.032.3476372
TATAACG4800.032.2172052
GGGTTAG13200.031.1209011
CATGGGG144100.031.0394844
TGGGGGG73750.029.517516
CGCATAG7150.029.1433161
GTTAAGA27750.028.2925174
CATGGGT66150.028.0533924
ATCACGC8750.027.8699653
CTATAGC11700.027.4516563
TAACGCA7200.026.4349274
GGTTAAG27850.026.268823
TCACGCA9450.025.8055254
GTGGTTA21100.025.1121881
TATGTAA23050.024.5136762
GGTTAGA15900.024.314872
CACTATA14150.023.5618061
ATATGTA22500.023.5496521
Produced by FastQC (version 0.11.5)