FastQCFastQC Report
Fri 10 Feb 2017
ERR1148286.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148286.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4472609
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT371340.8302536617889023No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA233200.5213959011395809No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT228610.5111334346463104No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207230.46333135760358213No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181490.40578105530798697No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG113790.25441526411094734No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92950.20782053606742731No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG91210.20393018929220058No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT80180.17926896806763123No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC79720.17824048558682415No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG75790.1694536678703638No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA73600.16455719692913018No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC68820.15386992245465678No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC68020.15208125727064448No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC64950.14521725462699736No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC58120.12994652561849246No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT56450.12621268704686683No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA51080.11420627199918436No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG5000.036.8781052
ATGGGGG100000.035.093685
CGCATAG6650.034.0164221
CATGGGG155900.033.8037224
TACAACG6800.033.2392772
TATAACG4950.032.444082
TGGGGGG85450.031.1148366
GGGTTAG15450.030.053341
TAACGCA7300.027.7033944
GTAAGTA34600.026.474122
CATGGGT56200.026.0360874
ACAACGC8800.025.6848963
GTTAGAG18650.025.5145593
ATCACGC8050.025.1223323
GTTAAGA32850.024.9873754
GGTTAGA17950.024.8527072
TCACGCA8200.024.6627754
TGTAAGT36450.024.6607361
GTGGTTA24950.023.3820251
GATTACT14350.023.2120253
Produced by FastQC (version 0.11.5)