FastQCFastQC Report
Fri 10 Feb 2017
ERR1148281.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148281.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3786931
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280150.739781105068986No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA251600.6643902410685593No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT243810.6438194939384954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158170.4176733085445708No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT141370.37331020818705174No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG111220.2936942870097184No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT99930.2638812273051714No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG97690.25796614725750217No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA90020.23771227941570627No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC86650.2288132527368468No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC84020.22186831500230658No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG77270.20404385503723202No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC73110.1930587063772749No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72560.19160634297271326No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC72360.19107821082559995No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT60060.15859808377813062No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG42620.11254496054984894No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC39350.10390999994454612No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT38920.10277451582825248No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCATAG4750.042.613041
TATAACG3300.037.852572
GGGTTAG13100.034.0837331
ATGGGGG66150.032.911455
CATGGGG111150.031.8422154
TACAACG5000.030.9309562
GTTAGAG15050.030.0381283
GGTTAGA14850.028.8400542
TTAGAGC16650.027.8660964
ATTAACG5550.027.8660953
TTAACGC4950.027.6387484
TGGGGGG56500.026.951776
TAGAGCA16750.026.6343575
CATGGGT49750.025.5868264
TAACGCA6850.024.314384
CACTATA11200.022.8563881
GTGGTTA18100.022.365961
ACGCATA2954.7439244E-922.1987151
AGTACTC30350.022.147035
GTTAAGA25900.022.04794
Produced by FastQC (version 0.11.5)