FastQCFastQC Report
Fri 10 Feb 2017
ERR1148267.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148267.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2991117
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279120.9331630959270399No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162560.5434758988030224No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140160.46858748755063745No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA126490.42288549729081143No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT113340.3789219880064872No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73890.24703146015351454No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG64040.21410061859833632No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG48400.16181246002747468No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT44120.14750342430603683No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG41110.1374402940439976No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC40060.13392989976654207No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC36150.12085786012382665No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC34300.11467287973021449No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA33450.1118311319817981No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC32930.11009265100629631No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC32900.10999235402694044No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA32330.10808671141917885No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA32260.10785268513401516No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31900.10664912138174468No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT31680.10591361019980161No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2850.043.8260462
CGCATAG4250.042.0246541
GGGTTAG10750.037.65931
TATAACG2250.037.008662
ATGGGGG63300.034.6715475
CATGGGG103000.033.4342734
TGGGGGG53350.033.1110236
GGTTAGA14700.028.3229522
GTTAGAG14550.028.2056873
TCACGCA4750.027.5473374
TACAACG4050.026.4347552
TTAGAGC16650.026.4343154
TAGAGCA16200.026.0671715
TAACGCA4500.025.1125984
AAACGCA8300.025.080755
CATGGGT33950.025.0522564
GTTAAGA20700.024.9976674
GTACATT12700.024.8452871
ACGCATA2451.6128979E-824.299971
TAGTACT7650.024.1018755
Produced by FastQC (version 0.11.5)