FastQCFastQC Report
Fri 10 Feb 2017
ERR1148265.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148265.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1373004
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153871.1206813672793379No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97460.709830415643363No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA88200.6423870578672749No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT57200.4166047586168722No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42440.3091032509737772No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT40890.29781413601125706No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG37530.27334224809250374No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC31910.23241010222839845No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30970.22556380025112818No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT25570.1862339803817032No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.18397615738919915No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC24680.1797518434032239No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA19250.1402035245345243No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC18030.13131789856402457No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC17610.1282589125741804No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA16890.12301493659159042No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA15560.11332814762375054No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15560.11332814762375054No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14920.1086668356392261No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1550.065.237842
TACAACG2400.052.046362
GGGTTAG5850.049.8675581
GTTAGAG7150.041.5954933
GGTTAGA7200.041.3066332
TTAGAGC7450.040.7189144
GGTTTCA7400.040.226861
TATCACG1508.54925E-1139.654372
GCGCAGA5450.039.3263741
ACAACGC3050.039.0042953
GTGGTTA12050.038.0435941
GTTAAGA14900.037.5252724
CATGGGG51300.036.8716054
TAGAGCA7750.036.840195
ATGGGGG32200.035.8367165
GTACAAC4500.035.7214551
GGTTAAG15300.035.377923
TTCAACG8650.034.38244
TAACGCA3000.033.7062154
TTAACGC1953.8198777E-1133.5536964
Produced by FastQC (version 0.11.5)