FastQCFastQC Report
Fri 10 Feb 2017
ERR1148262.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148262.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3434149
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284490.8284148416390786No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA237030.6902146645355225No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT228470.6652885474683831No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT162990.47461539962302157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145530.42377310943701046No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG110640.3221758869519057No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG96830.2819621396741958No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT93230.2714791932440905No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC80410.23414825623465957No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA73580.21425977731309853No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73140.21297852830497455No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC70400.20499984129983875No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG70140.20424273961322004No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC69810.20328180285712705No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC69150.20135992934494107No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT55740.16231095389279845No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG41480.12078683831132546No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC39340.11455530904454059No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA37960.11053684624633352No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT36700.10686781499579665No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA34640.1008692400941252No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3600.046.2602422
TATAACG3800.040.69512
GGGTTAG13650.036.6377831
CGCATAG5000.035.721841
TACAACG5500.034.6050642
ATGGGGG58850.032.340715
GGTTAGA15400.031.2835962
TTAGAGC16950.030.5278054
GTTAGAG15850.030.019733
CATGGGG99800.029.5595074
TAGAGCA17450.028.9714035
ACAACGC6650.028.6203123
TCACGCA5850.028.4674264
TAACGCA6500.028.3657574
GCGTATC2905.456968E-1226.6887321
CATGGGT38250.025.6565674
TGGGGGG52400.025.3116026
GTACAAC10950.024.4670141
ATCACGC6600.024.3313333
AGTACTC29200.024.2387475
Produced by FastQC (version 0.11.5)