FastQCFastQC Report
Fri 10 Feb 2017
ERR1148259.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148259.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4468023
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT393880.8815532059705153No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA265040.5931930072875632No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT255170.5711027002322951No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223360.4999079011007777No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206090.4612554590699287No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG118110.26434510296835984No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG105020.23504802907236602No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97600.21844113156982403No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC90060.20156565890551595No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT85080.1904197896922196No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG83420.18670449995445412No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA82810.18533924288214274No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC80320.17976630827549456No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC79490.17790866340661182No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70290.15731790100453827No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT58930.13189278569067348No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45720.10232713663291348No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA45500.10183474883634215No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC45230.10123045472236825No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4700.049.3550452
TATAACG4150.037.264052
ATGGGGG87850.034.597055
CATGGGG143000.033.6074264
TGGGGGG72400.031.053346
GGGTTAG12600.029.300641
CGCATAG6900.027.6157471
TCACGCA8650.026.8168934
TAACGCA6850.026.0489754
CTATAGC12000.025.7740143
ATCACGC9050.024.9743943
GTATCAC12400.024.4908851
CATGGGT45300.023.7651354
TTAACGC4600.023.274194
CACTATA14350.023.2376421
GTTAGAG16700.022.7941533
ATATGTA22600.022.6592731
GGTTAGA14750.022.5819172
GTTAAGA30900.022.3284954
TACAACG6750.022.0293182
Produced by FastQC (version 0.11.5)