FastQCFastQC Report
Fri 10 Feb 2017
ERR1148243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3485074
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT263080.7548763670441431No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA176470.5063594058547968No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT175110.5024570496924886No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138600.39769600301170077No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT132060.3789302608782482No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG84440.2422904076068399No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG68940.19781502487465114No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62800.1801970345536422No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC61300.17589296525697878No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT61090.17529039555544587No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG55100.15810281216410324No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA53000.15207711514877445No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC51010.14636704988186766No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC49180.14111608533993827No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC46080.13222100879350052No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT38480.11041372435707249No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACAACG4750.038.818872
TATCACG3300.034.246372
GGGTTAG10850.033.4742851
ATGGGGG68600.032.6015745
CATGGGG109300.032.3796164
TGGGGGG56400.029.1066576
TATAACG4100.029.0148932
ACAACGC6650.026.8333233
GGTTAGA13250.026.485672
GTTAGAG14100.025.732713
TAACGCA5600.025.4916574
CATGGGT44800.025.3588894
TTAACGC3900.024.4022674
TTAGAGC16200.023.8656464
GTACATT16150.023.2262571
AACGCAT3506.548362E-1122.0921346
AACGCAG592200.021.6944036
GTACACT42900.021.3734511
GTATCAA604000.021.1742761
AGTACTT210550.021.17297612-13
Produced by FastQC (version 0.11.5)