FastQCFastQC Report
Fri 10 Feb 2017
ERR1148242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3337457
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT259630.7779276257342043No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA193260.5790636403704976No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT191980.5752283849649599No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT144060.4316460107201381No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126700.3796303592825316No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG86800.2600782571880327No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG77650.2326621736250085No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT69910.20947086359464706No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC67220.20141083465644652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65210.19538828515243795No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG58860.1763618227890277No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA53600.16060132010689576No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC53410.16003202438263625No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC52710.15793461908273276No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC52010.15583721378282925No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT43970.1317470157667949No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG14200.033.542151
TATAACG3350.030.186262
ATGGGGG58550.029.0565665
TATCACG3500.028.8925632
GCGGTTA7500.028.5779131
CATGGGG96900.028.2997344
GGTTAGA16200.027.9063972
GTTAGAG17500.027.5329113
CGGTTAA8000.026.0245512
GTTAAGA24250.026.0015544
TTAGAGC19450.025.6900444
TACAACG5350.025.5728572
TAACGCA4450.025.3979574
GTACATT14600.024.875181
GGTTAAG23250.024.561423
TGGGGGG50800.023.6519416
CATGGGT40400.023.5582924
TAGAGCA21150.021.9376145
ACAACGC6550.021.7959143
GTATAAC7400.021.7230741
Produced by FastQC (version 0.11.5)