FastQCFastQC Report
Fri 10 Feb 2017
ERR1148238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3563163
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT331990.9317283548352966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177060.4969180472518378No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT168070.47168765504132143No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT154100.4324809165339896No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA154040.43231252681957016No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81540.22884162189605134No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG76980.21604400360017212No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG58270.16353447765370263No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC52510.1473690650694341No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG48720.13673244810860463No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT47170.13238238048610182No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC44400.12460838867040323No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA43640.12247545228775669No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC41950.11773247533160847No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA41670.11694665666431762No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41000.11506630485330029No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC39150.10987428865870015No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA37730.1058890654174395No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3350.039.0637442
GGGTTAG12850.031.9746191
ATGGGGG62000.031.9483595
TATCACG3800.031.3070662
CATGGGG109800.030.1209344
TACAACG5200.028.5978012
TGGGGGG52400.028.0381576
CGCATAG6950.027.4172921
TAACGCA5850.026.4370784
GGTTAGA15400.026.265412
GTTAGAG16200.025.3355333
ACGCATA4800.024.7840657
AACGCAG611600.024.284826
CATGGGT42550.023.6256164
GTATCAA628800.023.532781
TAGAGCA19000.023.167235
GTATAAC8850.022.8767871
ACAACGC6800.022.7436623
ACGCAGA673100.022.0306077
CGCAGAG674800.022.0279948
Produced by FastQC (version 0.11.5)