FastQCFastQC Report
Fri 10 Feb 2017
ERR1148234.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148234.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1112765
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99640.8954271566772859No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA80330.7218954586098592No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT74660.6709413038691907No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.5239201448643694No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG40190.3611723948902059No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG36780.33052800905851637No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33140.29781669984228476No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT33090.2973673686717321No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC31270.2810117140636163No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT23680.21280324237372672No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC22580.2029179566215688No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC21250.19096574748486878No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21130.18988735267554246No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC20330.18269805394670033No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA20080.18045139809393718No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG19140.17200397208754767No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG13290.1194322251328897No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA12540.11269225757460022No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC11710.10523336014342652No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA11480.1031664367588844No Hit
GTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTAC11450.10289683805655282No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAAAAAAAAA11230.10091978090612125No Hit
GGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACG11150.10020085103323703No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG1250.057.106562
TATCACG1451.8189894E-1245.127312
TAACGCA2250.042.3011554
GGGTTAG5150.041.62361
GTTAGAG5400.039.6573333
GGTTAAG6500.038.4371073
GGTTAGA6000.037.674472
GTTAAGA7000.035.69164
TACAACG1503.7252903E-935.69162
TTAGAGC6200.035.499714
GTGGTTA4750.033.8465581
TAGAGCA6900.033.622525
CGTATCA1451.10003384E-732.8198622
GAGCGCC3100.032.6184239
AACGCAT1659.451469E-932.4439936
ATGGGGG22400.032.1330645
GCGGTTA3350.031.9942571
TGGGGGG18900.031.785966
CATGGGG36850.031.6397744
ACGCATA1551.9763502E-730.6996947
Produced by FastQC (version 0.11.5)