FastQCFastQC Report
Fri 10 Feb 2017
ERR1148225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4114393
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338640.8230618708519094No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT287960.6998845273166662No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA286140.6954610315543508No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183390.4457279603577004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171330.41641622470191836No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG124250.301988653004222No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG115260.28013852833212577No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC101380.24640329691402837No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT96950.23563621656949155No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87360.21232779659113749No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC84990.20656753013141912No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG84640.2057168578694354No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA82480.20046699476690732No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC77690.18882493723861576No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC75970.18464449069401004No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT62680.15234324966039947No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG49900.12128155963710807No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC44230.10750066899297174No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC43320.10528892111181405No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT42310.10283412401294674No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG5350.048.9193152
TATAACG4000.038.6629372
GGGTTAG17300.036.826251
ATGGGGG85500.035.1323135
ATCACGC7800.033.5536353
CATGGGG133750.033.3096054
GTTAGAG21150.032.3421553
TGGGGGG75300.031.9908456
TCACGCA8550.031.3060254
GTGGTTA25800.031.1554361
GGTTAGA20500.031.0464122
TTAGAGC22500.029.8729024
GTTAAGA32150.029.6019634
GGTTAAG29700.029.2400363
TAGAGCA23700.028.1093735
TACAACG5950.025.9918922
ATATGTA20750.024.964391
GTATCAC13900.024.8446561
TATGTAA21900.023.9012182
AGTACTC31450.023.4521455
Produced by FastQC (version 0.11.5)