FastQCFastQC Report
Fri 10 Feb 2017
ERR1148223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2914480
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT216750.7437004199720019No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA129980.4459800719167742No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT124180.4260794378413988No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT121160.41571738354697924No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103520.35519200680738927No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG67680.23221981279679393No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG55640.19090884137135955No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54350.1864826658614916No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT50520.17334138508413166No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC47460.16284208503746808No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG44300.15199967061019462No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC39960.13710850649172407No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA38890.13343718261919793No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC38400.13175592215420934No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC37150.12746699239658532No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC36250.12437896297109603No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT35770.12273201394416844No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA30710.10537042628530648No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29340.10066975927095057No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG12400.044.169641
TATCACG2400.039.6532552
GTTAGAG15600.035.459163
ATGGGGG63400.035.3689545
CATGGGG97000.033.7870254
TTAGAGC15850.033.7740674
TATAACG2300.033.6190642
GGTTAGA16350.033.4688032
TAGAGCA15950.033.1894045
TGGGGGG53500.030.5727256
GTTAAGA20400.027.6989674
CTATAGC7350.027.5145033
GGTTAAG20050.025.8092273
GCGTATC1859.222422E-725.7441
ATATGTA18350.025.6300071
GTGGTTA14000.025.5141411
TATGTAA19400.025.4475782
GTACATT12150.024.4991721
CATGGGT38000.024.4180584
CACTATA9550.023.6885221
Produced by FastQC (version 0.11.5)