FastQCFastQC Report
Fri 10 Feb 2017
ERR1148222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4659721
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT372890.8002410444745511No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA268240.5756567828846405No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT240560.5162540847402667No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT200290.42983260156563025No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187860.403157184732734No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG127610.273857597911978No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG107690.23110825733987078No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT102810.22063552732019795No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97940.21018425781286046No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC90180.1935309002405938No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA87020.1867493783426089No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG84390.18110526359839998No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC80090.17187724329418005No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC76750.16470943217415807No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC72470.15552433289460893No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC67550.1449657608255945No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT64390.13818423892760962No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51970.1115302826070488No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4300.042.8815272
GGGTTAG20400.041.148841
TATAACG4950.038.4522552
ATGGGGG109800.036.132715
GGTTAGA23100.035.5340042
TTAGAGC25400.034.189774
CATGGGG163750.034.1447224
GTTAGAG24650.034.0235183
TGGGGGG92800.032.9444926
TAACGCA6900.031.8955174
TACAACG6350.031.8480052
TAGAGCA27300.031.3745045
ACAACGC7250.029.5353073
GTTAAGA34150.027.519684
GTACATT22150.027.1466541
GGTTAAG32700.026.557193
GTGGTTA26050.026.5105611
TCACGCA7300.026.0737884
CGCATAG8350.025.6675661
TGTAAGT44400.025.6105581
Produced by FastQC (version 0.11.5)