FastQCFastQC Report
Fri 10 Feb 2017
ERR1148221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3190564
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276640.867056733543035No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA234810.7359513866513883No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT225240.7059566897890154No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT155860.48850297314205265No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT137830.4319925881442904No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG110860.3474620788048759No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG98670.3092556676499829No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT85810.26894931429051416No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC80090.2510214494992108No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG73990.23190257271128242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70410.2206819860062359No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC70340.22046258905948918No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA69150.21673284096479492No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC68750.21547914412624225No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC66880.20961811140600847No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT54020.16931175804653975No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG40630.1273442563759887No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT37910.11881911787383047No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC36920.11571621819841257No Hit
GTATCAACGCAGAGTACATGGGCTTTTGTGATGAAGATGGGAGGGGGAAA33320.10443294665143843No Hit
CATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCA33030.10352401644348774No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3650.039.1121442
TACAACG5100.036.1563642
ACGCATA3000.035.6875957
ATGGGGG59300.033.50315
CATGGGG99650.031.7561284
TAACGCA5650.031.583924
ACAACGC5800.030.7670963
GGGTTAG9650.029.6162011
TGGGGGG50700.029.094426
AACGCAT3700.028.9358846
TATCACG3800.028.1761822
GTTAGAG12300.027.565323
GTTAAGA20950.027.5410794
GGTTAAG20800.026.309813
CGCATAG4600.025.887351
GTAAGTA22650.025.7365952
GTGGTTA18200.025.517531
AGTACTC25600.025.3267675
CATGGGT38800.024.9890144
TTAGAGC14050.024.9786474
Produced by FastQC (version 0.11.5)