FastQCFastQC Report
Fri 10 Feb 2017
ERR1148220.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148220.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3882615
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT314980.8112573613402307No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA176520.45464203893509914No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169140.4356342310530403No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT166940.42996794686055667No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160730.41397357193540946No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG85980.2214486885771574No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79110.2037544283942652No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG72250.18608592404861157No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC61570.1585786898778272No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT60330.1553849660602455No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG57350.14770972656315395No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA53530.13787099673802322No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC52960.13640291401542518No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC51590.13287436431374217No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC50280.1295003496354905No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT41800.10765939965718979No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG3650.037.4804572
GGGTTAG15900.036.6903081
TACAACG5950.034.988152
ATGGGGG75250.033.9094475
CATGGGG120200.032.2636154
ACAACGC7250.031.1756483
GTTAGAG18450.030.9488683
CGCATAG6150.030.0060621
GGTTAGA19000.029.4268782
TGGGGGG65100.029.0528076
TTAGAGC21550.027.3248544
TAGAGCA21250.027.1508055
GTGGTTA17550.027.1353421
TATAACG4200.026.9075552
CATGGGT56550.026.5056154
GTTAAGA23700.025.0969874
GGTTAAG22400.024.6947633
GTAAGTA32250.023.7919452
AAGAGCG12100.023.1025567
TGTAAGT33550.022.711191
Produced by FastQC (version 0.11.5)