FastQCFastQC Report
Fri 10 Feb 2017
ERR1148219.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148219.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4066040
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284550.699820956016173No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT217800.5356563142517043No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA216340.5320655969936351No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT151560.37274596413217775No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140600.34579099074283576No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG107300.2638931245142694No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG88210.2169432666673225No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC77890.19156230632261365No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT74020.18204444619334784No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA73640.1811098759480969No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71460.1757483940148154No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG69590.17114932465002805No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC67510.16603378225497042No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC66140.1626644105813027No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC63600.15641754631041505No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT49580.12193682305142103No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC49020.12055956163736707No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG4550.037.9117282
GGGTTAG15100.034.7022481
ATGGGGG80500.034.0637175
CATGGGG129100.031.7453384
TATAACG3950.031.6234472
TGGGGGG69300.031.2424076
TCACGCA5750.031.0341874
GTTAGAG17700.030.91733
GGTTAGA17200.029.0494442
CGCATAG6000.028.7805181
TTAACGC4850.026.9816134
TACAACG6100.026.3281842
TTAGAGC20350.025.4297334
ATTAACG5150.025.4098683
CATGGGT50850.024.798874
TAGAGCA20700.024.712415
ATCACGC7350.024.2784463
GTAAGTA31700.022.7045612
TAACGCA6750.022.0304414
ACAACGC7300.022.0002653
Produced by FastQC (version 0.11.5)