FastQCFastQC Report
Fri 10 Feb 2017
ERR1148215.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148215.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4080093
Sequences flagged as poor quality0
Sequence length125
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT399800.9798796252928548No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA269200.659788882263223No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT250140.6130742608072904No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206250.5055031833833199No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202290.4957975222623602No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG129660.31778687397566674No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG106730.261587174606069No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102890.2521756244281687No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC89500.21935774503179217No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT88710.21742151465665122No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA88320.2164656540917082No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG86900.21298534126550547No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC82900.20318164316352594No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC75060.18396639488364605No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC73920.18117234092458187No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC63690.1560993830287692No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT61210.15002109020554188No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT49850.12217858759591999No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45800.11225234326766571No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG43830.1074240219524408No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG42260.10357607044741382No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT41890.1026692283729807No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT40900.10024281309274077No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3300.048.6653062
TATCACG3900.042.703462
ATGGGGG102350.039.343275
CATGGGG153600.037.213614
TGGGGGG85500.036.521356
CGCATAG5300.034.8225141
GTGGTTA21300.034.1000141
GTTAAGA29850.033.2768144
GGTTAAG29550.032.6082233
TACAACG5700.032.348672
GTACATT20900.030.7646351
GGGTTAG17000.030.4680481
CATGGGT56850.027.7258594
TACATTG21250.027.4306932
GTTAGAG18850.027.136683
ATATGTA24650.026.8089831
TTAGAGC20050.026.1058544
ACAACGC7350.025.8959753
ATTAACG5750.025.8607883
TAACGCA7200.025.6093654
Produced by FastQC (version 0.11.5)