FastQCFastQC Report
Fri 10 Feb 2017
ERR1148198.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148198.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2334535
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT194200.8318573077722116No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114270.489476491035688No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93840.40196441689672674No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA57240.24518801388713385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52030.22287093575380107No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT49970.21404690869916276No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG31320.1341594792967336No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA28460.12190864561893482No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27450.11758230225719468No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG23530.10079094980370823No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG11400.036.041131
TATAACG1703.274181E-1034.9904252
TACAACG4100.034.819742
TATCACG2005.2750693E-1132.716052
GTTAAGA14950.031.8307514
ATGGGGG47100.031.6993945
GTTAGAG13050.031.4511643
GGTTAGA12700.031.3811762
TAACGCA3250.031.114564
TAGAGCA14000.031.0165145
CATGGGG78550.030.6695214
ACAACGC5000.029.7418613
TTAGAGC14500.029.5367454
GTGGTTA11550.027.8397881
GTATCAA297950.027.759591
GTATACG1306.347389E-527.4828681
GGTTAAG14200.027.2284643
AACGCAG313100.027.052446
CGCATAG3100.026.891841
GATTACT7700.025.4930253
Produced by FastQC (version 0.11.5)