FastQCFastQC Report
Fri 10 Feb 2017
ERR1148190.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148190.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2817524
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191000.6779001705043152No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA155030.5502348870852565No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT144920.5143523178507087No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114020.4046815572822095No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100290.3559508277480511No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG73110.25948314903440045No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG70790.25124896895288207No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT68150.24187903989460247No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC54340.19286437311625385No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51730.18360092052454566No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA49630.17614756786455057No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG48910.17359213266683798No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC48580.17242089153455303No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC47610.16897815244874578No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC47260.16773592700541326No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT39060.13863235947590866No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC34020.12074431309192042No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG29830.10587309992745404No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT29000.10292725101897979No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28280.1003718158212672No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2050.052.232882
TATAACG3250.042.0988162
GGGTTAG11200.039.8678931
TAACGCA4050.035.2518234
ATGGGGG66500.034.2611925
TAGAGCA14000.033.1430055
TTAGAGC14300.032.4477044
CATGGGG98900.032.420364
GGTTAGA13600.032.3681762
TGGGGGG54950.032.2578476
GTTAGAG14550.030.2547863
TACAACG4350.027.3505542
GTACGTT1608.706185E-626.0470241
CATGGGT36350.025.5296924
ATGGGTG21100.025.0918645
TCACGCA4650.024.30674
GTTAAGA23600.023.946224
ACGCATA2802.6211637E-923.3679967
AAGAGCG10350.022.988287
AGAGCGC11150.022.9393148
Produced by FastQC (version 0.11.5)