FastQCFastQC Report
Fri 10 Feb 2017
ERR1148189.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148189.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3384189
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA233030.6885844732667118No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217150.641660380079245No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT212790.6287769388766408No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT122230.36117959132897126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111450.32932557844730304No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG98240.29029111553757786No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT97700.28869545997578744No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG95660.282667427853468No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC77250.22826739286724235No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA67430.19925010098431264No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC66780.1973294044747501No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC65820.19449268347601154No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC65350.19310387215371244No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG65180.1926015361435192No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59550.1759653494530004No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT51560.15235555697391606No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG41800.1235155601534075No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC36410.10758855371257338No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT35490.10487002942211562No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC34350.10150142323611358No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG3250.043.927782
GGGTTAG12250.035.9638751
TAACGCA5200.030.886724
TATCACG3200.029.7427652
ATGGGGG66600.029.653455
TTAGAGC16600.029.0260754
GTTAGAG15000.028.9496273
TAGAGCA16650.028.2243675
CATGGGG104750.028.1668974
GGTTAGA15300.027.2156032
TACAACG4250.026.5935332
TGGGGGG55950.026.4714986
GTACATT16500.025.978821
GTGTTAT12600.024.5699161
ATATGTA16150.023.5927371
CATGGGT37550.023.4456984
GTTAAGA21450.023.2950344
GTAAGTA25150.023.1792552
TATGTAA17550.023.0485272
GGTTAAG21550.022.9109043
Produced by FastQC (version 0.11.5)