FastQCFastQC Report
Fri 10 Feb 2017
ERR1148185.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148185.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2397535
Sequences flagged as poor quality0
Sequence length125
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT208980.871645252311228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT123320.5143616255862792No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94040.3922361925894721No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA84870.35398857576636No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT72180.301059212899916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55260.23048672907799053No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG42580.17759907571735137No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC39100.16308416769723902No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT35650.14869438819454148No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG34460.14373095700375593No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33260.1387258163071655No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA32620.13605640793565058No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC27400.11428404590548208No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC26840.11194831358040654No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG26360.10994625730177036No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG25790.1075688154708899No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT25100.10469085957035038No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG2650.044.8913572
TACAACG3350.042.6132852
GGGTTAG10150.039.2961271
ATGGGGG67450.035.6268965
GTTAAGA19550.035.29314
GCGTATC2250.034.395521
CATGGGG96250.034.0509644
TGGGGGG56750.033.2255366
GTTAGAG12100.032.9357873
GGTTAAG19450.032.41643
GGTTAGA11900.031.9898072
TAGAGCA13950.031.9790535
TATAACG2255.456968E-1231.7232232
TCACGCA4150.031.532124
GTGGTTA15200.031.3319511
TTAGAGC14000.030.590254
ACAACGC4300.030.4321613
AGAGCGC8200.029.7405228
TAACGCA3250.029.2829724
CGCATAG3300.028.8633731
Produced by FastQC (version 0.11.5)