FastQCFastQC Report
Fri 10 Feb 2017
ERR1148184.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148184.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2705141
Sequences flagged as poor quality0
Sequence length125
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT186660.6900194851211082No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA167200.6180823846150718No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT163590.6047374240381556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT111200.41106914574877984No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT96510.3567651371961757No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG79990.29569623173061954No Hit
GCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGT77510.2865285025808266No Hit
GAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGG72500.26800821103225303No Hit
GCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCC59690.22065393264158872No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC59420.21965583309705483No Hit
ACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGC55540.2053127729756046No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG54580.20176397459503959No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51710.19115454610314214No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC51280.18956498016184736No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATAC50530.18679248142703098No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA49250.1820607502529443No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACAT45290.16742195693311365No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG43230.15980682707481791No Hit
GGTCCAGAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT33840.12509514291491644No Hit
ACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGATACCACTG32270.11929137889670077No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30310.11204591553638055No Hit
AAAAAGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT30100.11126961589063196No Hit
CTGCTTCCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGC29590.10938431675095678No Hit
CAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGT28540.10550281852221381No Hit
GGTCCGAAGTTCAAATCCCAGCAACCACATGGTGGCTCACAACCATCCGT27700.10239761993921943No Hit
GTATCAACGCAGAGTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAG27120.10025355425096141No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATAACG2700.052.870652
GGGTTAG14950.046.5913621
GTACATT14000.044.2248151
TACAACG3500.044.184762
TAGAGCA16750.041.191765
TACATTG14300.041.1781042
GGTTAGA16400.040.9828152
TAACGCA4100.040.6201364
GTGGTTA16800.038.9802061
TTAGAGC17250.038.963374
GTTAGAG17100.038.957323
ACAACGC3950.037.645243
GTTAAGA24900.036.308764
ATGGGGG90950.036.230495
GGTTAAG23400.036.0943833
CATGGGG121300.035.3542564
TGGGGGG76600.034.554016
AAACGCA6700.032.8468785
GTACAAC6450.031.3819481
TTGTAGC16900.030.2676686
Produced by FastQC (version 0.11.5)