FastQCFastQC Report
Fri 10 Feb 2017
ERR1148175.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148175.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences783944
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33160.422989397201841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18940.2415988897166124No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.20703009398630512No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA22350.058.6154631
GGTATCA11200.054.7628751
TCAACGC28850.044.9533424
CAACGCA29500.044.1645135
AACGCAG29700.044.0674136
ATCAACG29700.043.4664963
TATCAAC31800.041.1599242
ACGCAGA33200.039.600947
CGCAGAG34300.038.330948
GCAGAGT38800.033.885349
CGTATCA902.3935293E-433.0526662
GTACATG35150.033.0351521
TACATGG35900.031.6532232
ACATGGG35350.031.3021983
GTACCGT1203.6983452E-529.7455026
CATGGGG20150.029.5240734
GTATATC1055.876185E-428.356181
AGAGTAC35450.028.1092910-11
GAGTACT18350.026.9087412-13
GTATTAC1556.785065E-626.8926351
Produced by FastQC (version 0.11.5)