FastQCFastQC Report
Fri 10 Feb 2017
ERR1148174.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148174.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences711274
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26610.37411742872648235No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13450.1890973098974516No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11470.16125993639581934No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA18700.065.904611
GGTATCA8600.056.767851
TCAACGC25850.047.3986634
CAACGCA26250.046.67645
AACGCAG26600.046.509446
ATCAACG26700.045.8897173
TATCAAC27750.044.3676832
ACGCAGA31650.038.9005787
CGCAGAG33350.037.0959858
GTACATG35450.036.7802731
GCAGAGT35400.034.9477739
TACATGG37700.034.866662
ACATGGG37300.034.7621883
GTATAGA1606.9285306E-933.489571
CATGGGG22700.030.9182974
AGAGTAC33950.028.29385810-11
ATGGGGG9700.026.97995
CAGAGTA35300.024.17890410-11
AGTACTT15750.023.79136712-13
TGGGGGG6950.023.1067246
Produced by FastQC (version 0.11.5)