FastQCFastQC Report
Fri 10 Feb 2017
ERR1148173.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148173.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1144877
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43170.37707107401057055No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25140.21958690758919955No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.18263970714758004No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA27700.068.162821
GGTATCA11750.067.9258351
TCAACGC38100.049.183814
CAACGCA39350.047.621435
ATCAACG39800.046.9335253
AACGCAG41500.045.2976466
TATCAAC43100.043.3437962
ACGCAGA48400.038.59417
CGCAGAG50300.037.254548
GCAGAGT56300.033.2842529
TGCGACG800.00536704629.7444975
GTACATG50300.029.366471
AGAGTAC52000.028.1428710-11
TACATGG52150.027.836212
ACATGGG51500.027.2609773
AGTACTT23850.025.44183512-13
ATGGGGG13900.024.3947685
CAGAGTA55050.024.26027110-11
CATGGGG29100.023.7138254
GTCTAGT2302.3400935E-723.3068031
Produced by FastQC (version 0.11.5)