FastQCFastQC Report
Fri 10 Feb 2017
ERR1148172.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148172.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1007285
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43870.4355271844612002No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22930.22764163072020333No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21710.21552986493395612No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA29550.064.6711351
GGTATCA13450.063.738691
TCAACGC38850.049.3040584
CAACGCA39200.048.7120975
ATCAACG39600.048.5204933
AACGCAG40800.046.801826
TATCAAC43100.044.4444962
ACGCAGA47150.040.3725477
CGCAGAG48600.039.1680158
GCAGAGT52750.036.086559
TAACCGT750.00391462931.7260936
GTACATG48650.030.5928631
TACATGG50450.029.3614562
ACATGGG50500.029.3309293
AGAGTAC49350.028.9295710-11
CATGGGG28100.027.097024
CAGAGTA51850.026.6742410-11
GAGTACT22450.026.49729212-13
ATGGGGG14700.024.280175
GTATAGA3201.8189894E-1124.1855681
Produced by FastQC (version 0.11.5)