FastQCFastQC Report
Fri 10 Feb 2017
ERR1148170.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148170.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences774094
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28080.36274664317253463No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15790.20398039514580918No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.1879616687379052No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8550.066.85941
GTATCAA18350.061.980441
TCAACGC24700.045.276124
ATCAACG25000.044.73283
CAACGCA25400.044.028355
AACGCAG25900.042.948716
TATCAAC28100.040.435552
ACGCAGA30350.036.4554567
CGCAGAG31300.035.3489768
GCAGAGT35450.031.3786059
GTACATG31700.029.8672491
CTTAGCG800.005367698629.7425563
TACATGG32100.028.9105222
ACATGGG32400.027.9065953
AGAGTAC31950.026.99637410-11
CAGAGTA33850.024.86600510-11
AGTACTT15300.024.49386812-13
GAGTACT14400.024.16582712-13
CATGGGG17450.023.5213344
TACACTA3052.746674E-1023.4039785
Produced by FastQC (version 0.11.5)