FastQCFastQC Report
Fri 10 Feb 2017
ERR1148168.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148168.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1390544
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64240.4619774706877309No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35910.2582442554856229No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32840.23616656502778768No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46550.059.596281
GGTATCA23800.056.2805671
TCAACGC61350.044.797344
CAACGCA62400.043.9482085
ATCAACG62400.043.8528753
AACGCAG63300.043.323356
TATCAAC65500.042.2330172
ACGCAGA74100.037.169587
CGCAGAG75900.036.3664638
GTACATG77750.033.4606171
GCAGAGT83950.032.8792659
TACATGG79900.032.387912
ACATGGG79050.032.0577133
CATGGGT19500.028.3709034
CATGGGG43000.027.9452324
AGAGTAC78650.027.11535510-11
GAGTACT34850.026.71384412-13
ATGGGGG24600.026.6000465
AGTACTT36650.025.32068412-13
CAGAGTA81500.025.2912610-11
Produced by FastQC (version 0.11.5)