FastQCFastQC Report
Fri 10 Feb 2017
ERR1148167.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148167.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1627368
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT72700.44673362140585293No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38590.23713136795119483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38380.23584094070916964No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA51400.063.7419551
GGTATCA23550.061.466881
TCAACGC67550.047.7264144
CAACGCA69250.046.46895
AACGCAG69650.046.4582256
ATCAACG69950.046.1739433
TATCAAC73500.043.9518782
ACGCAGA81050.039.8503047
CGCAGAG83850.038.5195858
TATACCG1401.899025E-938.2383655
GCAGAGT91150.035.565159
GTACATG82500.032.4925961
TACATGG83950.031.6775632
ACATGGG83800.031.3025283
AGAGTAC85250.029.23509410-11
CATGGGG51850.028.9092374
CAGAGTA87850.026.5755810-11
AGTACTT43050.026.52851512-13
AGCGTAA900.00954424326.4364036
GAGTACT41150.025.80199212-13
Produced by FastQC (version 0.11.5)