FastQCFastQC Report
Fri 10 Feb 2017
ERR1148166.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148166.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1671051
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57430.3436759261087783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29890.1788694659827857No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29770.17815135504541751No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42300.068.423241
GGTATCA18600.061.7948231
TCAACGC57500.049.5528144
CAACGCA58350.048.8309675
ATCAACG58650.048.6826173
AACGCAG59900.047.4680866
TATCAAC64050.045.5123862
ACGCAGA69300.041.029417
CGCAGAG72250.039.3541648
GCAGAGT79400.035.8852239
GTACATG74600.032.17171
TACATGG76250.031.5985982
ACATGGG77550.030.4515483
AGAGTAC73700.029.94381110-11
CAGAGTA76100.026.34182710-11
ATAGGGG2558.8584784E-1025.6597943
CATGGGG47350.025.376524
AGTACTT36350.025.03731212-13
GAGTACT33050.024.92751512-13
CATGGGT17900.023.9265524
Produced by FastQC (version 0.11.5)