FastQCFastQC Report
Fri 10 Feb 2017
ERR1148165.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148165.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1623914
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69230.42631567927858244No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35930.22125555909980454No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35540.21885395408870173No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18600.069.157091
GTATCAA43800.067.029931
TCAACGC58300.049.3815044
CAACGCA60550.047.6447535
ATCAACG60950.047.3320733
AACGCAG61200.046.9443366
TATCAAC62300.046.504532
ACGCAGA70850.040.4664087
CGCAGAG73350.039.0060928
GCAGAGT80600.035.349889
GTACATG76100.034.9016651
TACATGG77950.033.733432
ACATGGG78250.033.1428343
AGAGTAC75900.029.89787110-11
CATGGGG50500.028.975524
ATGGGGG31750.026.9777855
AGTACTT38850.026.71725812-13
CAGAGTA80250.025.86829810-11
CATGGGT14100.025.7334614
GAGTACT36550.025.3877812-13
Produced by FastQC (version 0.11.5)