FastQCFastQC Report
Fri 10 Feb 2017
ERR1148164.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148164.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2196680
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85450.3889961214195969No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45160.20558297066482148No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43130.19634175209862154No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA63600.063.6742671
GGTATCA29100.056.0750541
AACGCAG84650.047.225846
TCAACGC84700.047.1979644
CAACGCA86350.046.4338765
ATCAACG87450.045.645723
TATCAAC93300.043.1074032
ACGCAGA99700.040.0969627
CGCAGAG103600.038.7023628
GCAGAGT116500.034.7743159
GTACATG105750.031.5932731
TACATGG109350.030.4144422
ACATGGG108800.029.908583
AGAGTAC106350.029.87040710-11
CATGGGG64750.027.8381424
AGTACTT52100.025.7481612-13
CAGAGTA112800.025.15629610-11
GAGTACT48450.024.61829412-13
CGTATAG2001.8127066E-623.8216671
AGTACAT79900.023.08087512-13
Produced by FastQC (version 0.11.5)