FastQCFastQC Report
Fri 10 Feb 2017
ERR1148163.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148163.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2548233
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85980.3374102760618829No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44280.1737674694582481No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41650.16344659220722751No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA67550.061.711621
GGTATCA32350.053.0166741
AACGCAG87950.047.140776
TCAACGC87650.047.098524
CAACGCA89100.046.5323335
ATCAACG91250.045.3055763
TATCAAC98750.042.1097262
ACGCAGA105100.039.3918387
CGCAGAG109450.037.8262448
GCAGAGT120150.034.4081049
GTACATG117400.033.9353451
TACATGG122650.032.2549552
ACATGGG122150.031.9455283
CATGGGG69200.030.1717664
AGAGTAC113250.028.52077510-11
ATGGGGG36050.026.2356365
CAGAGTA118050.024.96763410-11
AGTACTT56250.024.3223212-13
GAGTACT51450.024.2213512-13
CATGGGT28850.024.123474
Produced by FastQC (version 0.11.5)