FastQCFastQC Report
Fri 10 Feb 2017
ERR1148162.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148162.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1865023
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT71550.3836413813663424No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37080.19881792342507304No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT36630.19640508454855515No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24400.065.660261
GTATCAA51800.064.616991
TCAACGC72700.045.738474
ATCAACG73700.045.1178673
AACGCAG73700.045.0371556
CAACGCA74200.044.7336655
TATCAAC79050.042.445152
ACGCAGA85950.038.5490387
CGCAGAG88600.037.396058
GTACATG94600.034.311961
GCAGAGT97550.033.9650429
TACATGG97150.033.4961852
ACATGGG98150.032.7271163
CATGGGG54950.032.259144
AGAGTAC90200.029.28064710-11
CATGGGT21450.028.8409984
ATGGGGG33000.026.6779215
GAGTACT39850.025.89854612-13
CAGAGTA95300.025.87234910-11
AGTACTT42150.025.1909712-13
Produced by FastQC (version 0.11.5)