FastQCFastQC Report
Fri 10 Feb 2017
ERR1148161.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148161.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences994413
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34180.34372036568307135No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.18010625363908153No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15940.1602955713571725No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25100.059.7932131
GGTATCA12100.054.6339651
TCAACGC32100.045.579044
CAACGCA32750.044.4928135
ATCAACG33800.043.4625633
AACGCAG34300.042.482216
TATCAAC36950.040.7272262
ACGCAGA40200.036.2472537
CGCAGAG41550.035.3558278
GCAGAGT45650.032.1803869
GTACATG42650.030.1619761
ACATGGG43950.028.2828043
TACATGG44900.028.0846022
AGAGTAC42450.027.46081510-11
GTTATAT2809.276846E-1125.5239731
GTATTAG5700.025.0761851
CATGGGG25450.025.0052414
TCTATAC3151.6370905E-1124.5452753
CAGAGTA44150.024.24805310-11
TATAGGG1501.6900148E-423.7924272
Produced by FastQC (version 0.11.5)