FastQCFastQC Report
Fri 10 Feb 2017
ERR1148160.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148160.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1126304
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55450.49231823734977415No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29840.26493735261527973No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26920.23901184760064778No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17900.060.8656271
GTATCAA36100.060.524741
TCAACGC49500.043.9848144
AACGCAG50200.043.6084826
ATCAACG50350.043.2422683
CAACGCA50750.042.9014435
TATCAAC53750.040.6194232
ACGCAGA58300.037.3455967
CGCAGAG59850.036.3784188
GTACATG62850.034.00661
ACATGGG63900.032.9556053
GCAGAGT65950.032.9234249
TACATGG65350.032.7720072
CATGGGG37500.029.8231474
CATGGGT16500.029.203034
AGAGTAC61650.027.93459510-11
GTATAGG2353.3833203E-1027.8675561
ATGGGTA8800.026.3638485
GAGTACT29150.025.815412-13
CAGAGTA63500.025.80921410-11
Produced by FastQC (version 0.11.5)