FastQCFastQC Report
Fri 10 Feb 2017
ERR1148159.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148159.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences874577
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27820.31809663414427775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16300.18637581367907No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14260.16305025172168947No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9600.061.4053461
GTATCAA19000.057.6642271
TCAACGC23600.045.625894
CAACGCA24500.043.9498375
AACGCAG25200.042.965086
ATCAACG25250.042.408793
TATCAAC27350.039.5920942
ACGCAGA29600.036.5783777
GTACATG33650.035.7444421
CGCAGAG31650.034.5850878
TACATGG35900.033.64312
ACATGGG34850.033.1164443
GCAGAGT35150.031.4798329
TATAGGG1403.094543E-629.7484552
CATGGGG21750.028.4458474
GAGTACT13550.027.87912812-13
GCGTTAC900.00949301726.4642561
AGAGTAC31150.026.3551710-11
CAGAGTA33550.025.00180610-11
ATGGGGG11750.024.3023415
Produced by FastQC (version 0.11.5)