FastQCFastQC Report
Fri 10 Feb 2017
ERR1148158.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148158.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1734680
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62000.3574146240228745No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31770.18314617105172135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30080.17340374017109783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21300.062.07211
GTATCAA43250.061.4146271
TCAACGC58300.044.793814
ATCAACG59500.043.9903873
CAACGCA59650.043.780045
AACGCAG62100.042.2443926
TATCAAC64000.041.1773452
ACGCAGA71900.036.56927
GTACATG81250.035.1836551
CGCAGAG75000.035.057678
TACATGG83950.034.0137982
ACATGGG83950.033.3042143
CATGGGG42450.031.9506174
GCAGAGT84550.031.3089499
AGAGTAC76400.027.6412110-11
GAGTACT35400.024.87027412-13
CGTTAAC1200.001289551324.7869642
AGTACTT37400.024.65370412-13
CAGAGTA81000.024.38232610-11
ATGGGGG22700.024.3713265
Produced by FastQC (version 0.11.5)