FastQCFastQC Report
Fri 10 Feb 2017
ERR1148157.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148157.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2753629
Sequences flagged as poor quality0
Sequence length125
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114120.41443491479789035No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58360.21193850006663933No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57220.2077985088041998No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA84450.059.0300641
GGTATCA42850.048.2309841
AACGCAG109800.044.700246
TCAACGC112350.043.8445364
CAACGCA114800.042.9088335
ATCAACG116200.042.3918573
TATCAAC123450.040.341842
ACGCAGA128050.038.422377
CGCAGAG132050.037.2134488
GTACATG132500.034.56671
GCAGAGT144300.034.1779869
TACATGG136450.033.6203462
ACATGGG134250.033.4129873
CATGGGG81550.031.9527634
AGAGTAC134500.029.74597710-11
ATGGGGG39900.029.224125
CATGGGT27400.027.1404914
AGTACTT70500.026.15958212-13
CAGAGTA139900.025.04700710-11
GAGTACT66000.024.74324412-13
Produced by FastQC (version 0.11.5)