FastQCFastQC Report
Fri 10 Feb 2017
ERR1148156.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148156.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2205797
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79340.359688584216952No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41030.18600986400833802No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39840.18061498859595873No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA58900.062.0839161
GGTATCA28300.054.0844841
TCAACGC79450.045.5960664
AACGCAG80050.045.1799966
CAACGCA80200.045.1696625
ATCAACG81600.044.3217963
TATCAAC89800.040.674812
ACGCAGA91700.039.3752487
CGCAGAG95650.037.749198
GTACATG100050.034.8824161
GCAGAGT105900.034.263989
TACATGG102950.033.6880342
ACATGGG102350.033.243913
AGAGTAC97700.029.8335910-11
CATGGGT22900.029.612384
CATGGGG61600.028.6800374
AGTACTT48800.025.9635312-13
GAGTACT45600.025.43745812-13
CAGAGTA101100.025.3588810-11
GTATATG6600.023.4614831
Produced by FastQC (version 0.11.5)