FastQCFastQC Report
Fri 10 Feb 2017
ERR1148154.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148154.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1570586
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56150.3575098721114285No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30120.19177555383786687No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28090.17885044180961757No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA42750.058.643691
GGTATCA21550.055.2659951
TCAACGC56200.044.032754
CAACGCA57300.043.1874475
AACGCAG57950.042.703036
ATCAACG58450.042.337733
TATCAAC63900.039.6640132
ACGCAGA65600.037.63257
CGCAGAG68850.035.942498
GTACATG75850.033.915911
GCAGAGT74050.033.5791749
ACATGGG76450.033.225323
TACATGG78050.033.006762
CATGGGG43750.031.5448674
AGAGTAC70400.028.64480210-11
CATGGGT19400.026.983594
ATGGGGG22800.026.873315
AGTACTT32350.026.38738612-13
CAGAGTA73100.025.14547710-11
GAGTACT30450.025.10351812-13
Produced by FastQC (version 0.11.5)