FastQCFastQC Report
Fri 10 Feb 2017
ERR1148153.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148153.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1264676
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50870.402237411004874No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25480.201474527863263No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23750.18779513488039623No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA36350.065.044541
GGTATCA17350.061.4438321
TCAACGC50350.046.5467034
ATCAACG51150.045.934993
AACGCAG51850.045.08546
CAACGCA52100.044.869065
TATCAAC54600.043.362762
ACGCAGA61250.038.1661727
CGCAGAG62600.037.34318
GTACATG70900.035.6999361
ACATGGG70550.035.1585663
TACATGG72350.034.862122
ATGGGGG29450.033.932525
GCAGAGT69250.033.7570849
CATGGGG46550.032.7124024
CATGGGT15000.029.7414514
AGAGTAC65950.029.17773810-11
TGGGGGG24750.028.3595246
TAGGCGA1358.262969E-526.4368444
CAGAGTA66350.025.63995410-11
Produced by FastQC (version 0.11.5)