FastQCFastQC Report
Fri 10 Feb 2017
ERR1148152.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148152.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1117972
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41740.37335460995445324No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21350.19097079354402435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20910.18703509569112645No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA32750.059.825561
GGTATCA16000.057.319571
TCAACGC43850.044.21874
ATCAACG44050.044.0179333
AACGCAG44350.043.7201776
CAACGCA44900.043.0521665
TATCAAC46300.041.8788342
ACGCAGA51800.037.432247
GTACATG57700.036.8463551
TATACCG1304.2160536E-836.6019825
CGCAGAG54250.035.8513878
TACATGG59600.035.427462
ACATGGG58500.035.2802433
GCAGAGT59650.032.7055449
CATGGGG37450.032.558174
ATGGGGG20100.031.9584455
GTATAGA2750.030.3177871
CATGGGT13700.029.9561844
TATGTAA6800.027.9897522
AGAGTAC55700.027.97718610-11
Produced by FastQC (version 0.11.5)