FastQCFastQC Report
Fri 10 Feb 2017
ERR1148151.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1148151.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1268085
Sequences flagged as poor quality0
Sequence length125
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51190.4036795640670775No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27300.2152852529601722No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25260.19919800328842308No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38450.058.411321
GGTATCA19150.054.7514841
TCAACGC49550.044.6616064
AACGCAG49850.044.3928346
CAACGCA50600.043.8524065
ATCAACG51100.043.423323
TATCAAC55100.040.4885182
ACGCAGA57900.038.220777
CGCAGAG60350.036.6691448
GCAGAGT65000.033.9543659
GTACATG61650.032.4681971
TACATGG63800.031.610492
ACATGGG65150.030.5890123
AGAGTAC59300.029.09232310-11
CATGGGG35050.027.1560764
TATACCG1107.7602227E-427.0403565
CAGAGTA63100.025.83189810-11
GAGTACT26750.025.68580812-13
AGTACTT29050.024.8808512-13
CATGGGT13900.024.394684
Produced by FastQC (version 0.11.5)